Biology-Talk

After some paper-reading, I realized there are two ways to quantify TUNEL: either by flow cytometry, or using chamber slides or cytospin to adhere cells to a glass slide, performing TUNEL with an in-situ kit, and then counting by direct microscopy.

When I presented this discrepency to a senior lab member, he just gave me a cold stare and asked me which was more accurate: counting 400 cells or 10,000? However, I think there must be some reason why so many papers use the glass slide method !

Does it have something to do with the adhesiveness of cells (all the papers using glass slides use adhesive cells)? After all that failure and disappointment I went through with annexinV/PI, I don’t want to make the same mistake again!

And another question: If I’m doing TUNEL via flow, is single staining enough or should I counter-stain it with PI or something? I’m not sure what the function of counter-staining in this situation actually is…. Anyways I’m a newbie in this apoptosis thing (my prof asked me to do this two months ago), any help at all would be greatly appreciated!

One cell with:
- malignant mutation,
- arrested apoptosis,
- impaired signaling
means that the cell will produce it’s similar cells, replacing the healthy cells. (as told in the link below, by the first answerer):

http://answers.yahoo.com/question/index;_ylt=AhL3MFZ85EfnY7SOw.b9kUq.DH1G;_ylv=3?qid=20100424211012AAQcxDB

(it is said that the cancerous mutation starts from ONE cell in most of the cases, then it makes other cells like itself by cell division and other mechanisms)

1. Can ONE such cell with such mutation be diagnosed as soon as the mutation occurs? If yes, how? If no, why?

2. Or is it means that the person who has developed ONE such cell is meant to develop cancer for rest of his life?

3. If a certain group of cells (at one place in body) are diagnosed with such a mutation, CAN they be cured, so that they have right cell functions, correct base sequence after the treatment? (also, producing healthy cells as before after cell division?)

I used to repair computers for nine years and have vo-tech training in electronics repair.

In a year, I will be graduating with a B.S. in molecular biology.
In addition to my degree I have taken two college level programming courses for computer science majors, calculus I through IV, digital logic and digital signals and filtering.

I can program up to mid level programs in Java, but I am far from being a whiz.

What careers are open to me? I am not looking into grad school until later, I just need a job first.
Also, can I do bioinformatics? I don’t have any formal bioinformatics education, just the stuff I listed above.
I am looking for ideas on what I can do, I am open to anything that sounds good.

A landmark paper on homeopathy and cancer has appeared in the February 2010 issue of the International Journal of Oncology. Scientists at the University of Texas M.D. Anderson Cancer Center (MDA), led by Moshe Frenkel, MD, have confirmed the ability of four homeopathic remedies to induce apoptosis (programmed cell death) in breast cancer cell lines in the laboratory. The scientists in question were from the Integrative Medicine Program, the Department of Molecular Pathology, and the Department of Melanoma Medical Oncology of MDA. Their two Indian collaborators were from the Banerji Homeopathic Research Foundation, Kolkata, India, where these same remedies are employed clinically with apparent success. The four ultra-dilute remedies in question were Carcinosin, Phytolacca, Conium and Thuja.

“The remedies exerted preferential cytotoxic effects against the two breast cancer cell lines, causing cell cycle delay/arrest and apoptosis” the authors wrote.

It was particularly interesting that the cell-killing effects of two of the remedies investigated in this study, Carcinosin and Phytolacca, appeared similar to the activity of paclitaxel (Taxol), the most commonly used chemotherapeutic drug for breast cancer, when it was tested in the same two adenocarcinoma cell lines investigated in this study.

http://www.cancerdecisions.com/content/view/414/2/lang,english/

University of Colorado, Boulder or University of Delaware

Specially the lesser known career options. Also any one year kind of courses which can help in boosting career.

I’m taking human physiology this upcoming semester. I have a choice whether to pick genetics or humany physiology and most students recommend human physiology. I was wondering is there any biochemistry or molecular biology involve in human physiology. Also is there any chemistry stuff in it?
Ok I really love biology but hate chemistry. I have took biology 1 and 2 with A and I also made an A in chemistry 1. I haven’t took chemistry 2 yet. What is biochemistry about? Is it a mix of biology and chemistry?

apoptosis refers to programmed cells death, and is a normal process during the full life span of the organism. Describe the roles of apostosis in the human body, and briefly discuss what can happen when the cells lose the ability to undergo apoptosis.