Biology-Talk

After some paper-reading, I realized there are two ways to quantify TUNEL: either by flow cytometry, or using chamber slides or cytospin to adhere cells to a glass slide, performing TUNEL with an in-situ kit, and then counting by direct microscopy.

When I presented this discrepency to a senior lab member, he just gave me a cold stare and asked me which was more accurate: counting 400 cells or 10,000? However, I think there must be some reason why so many papers use the glass slide method !

Does it have something to do with the adhesiveness of cells (all the papers using glass slides use adhesive cells)? After all that failure and disappointment I went through with annexinV/PI, I don’t want to make the same mistake again!

And another question: If I’m doing TUNEL via flow, is single staining enough or should I counter-stain it with PI or something? I’m not sure what the function of counter-staining in this situation actually is…. Anyways I’m a newbie in this apoptosis thing (my prof asked me to do this two months ago), any help at all would be greatly appreciated!

A. differences in cellular genomes.
B. apoptosis.
C. cell division.
D. morphogenesis.
E. differential gene expression.

1) differences in cellular genomes

2) morphogenesis

3) differential gene expression

4) apoptosis

5) cell division

Which one is correct? Thanks TONS!!

In patients with AIDS, circulating neutrophils show an impaired ability to be attracted by chemokines and are observed to undergo apoptosis at an increased rate. Which one of the following is the most direct consequence of these facts?

-Mechanisms of hemostasis are more likely to be defective in AIDS patients compared to healthy persons.
-The ability of dendritic cells to present antigens to lymphocytes is impaired.
-Bacterial infections that do not cause illness in normal persons are more likely to cause serious disease in AIDS patients.
-Tissue inflammation is more pronounced in AIDS patients.
-Fewer B-lymphocytes accumulate in damaged tissues.